Wednesday, April 10, 2013

Beer's Law

Beer's Law has to do with spectrophotometers, which were discussed in the previous post. Beer's Law is given by the equation A=ebc, where A is the absorbance (A has no units), E is the molar absorptivity with the units L/(mol*cm), b is the path length (cm), and c is the concentration of the solution (mol/L). 

This equation can be used not only to find the absorbance of a solution given the other three variables, but it can be applied to spectrophotemtry. The spectrophotometer could output the absorbance, and b (measure the size of the cuvette) and E (use a graph) are known, so the concentration of the solution could be found.

Beer's Law is useful in not only finding concentrations of solutions one creates. Researcher's apply Beer's Law to their research. In many cases, Beer's Law can be used to determine the concentration of plasmid, DNA, or protein in a solution, which can then be used for calculations to find out how much would need to be placed in a PCR or other experimental procedure.

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