The Use of Micropipettes

     Scientists use micropipettes when dealing with very small amounts of liquid. The samples micropipettes can take range from 0.01 uL (microliters, or 10^-6 of a liter) to about 1000uL (1mL). The following table shows different pipette sizes and their tips:

     In addition to these sizes, there is a P-2, which pipettes from 0.01uL to about 2uL for more precise measurements. It is important to know how to use a micropipette correctly, because measurement is key when it comes to research. When working with materials that can be easily contaminated, make sure you change tips each time to reduce cross contamination.

     The first thing to remember when learning how to use a micropipette is to hold it properly. Grip the pipette so that the ledge rests on your index finger, and you can press down the plunger with your thumb. Remember, the plunger is different from the tip eject button. If you press this one by accident, your tip will be pushed off the pipette. To adjust the volume you want to pipette, turn the black knob at the top so that it does not make a right angle. Then you can twist the plunger portion of the pipette, and you will see the numbers on the side change. This number indicates the amount of liquid you are pipetting. When you are done, twist the black knob back to lock the measurement. To pipette, push the plunger until you feel an initial stop (not all the way down), and then insert the tip into the liquid you wish to pipette. Let go of the plunger completely. Then push the plunger completely down to the last stop into whatever container you want the liquid in. That's all there is to it!